Inhibition of H2O2-induced apoptosis of GC2-spg cells by functionalized selenium nanoparticles with lentinan through ROS-mediated ERK/p53 signaling pathways

A H2O2-induced oxidative stress injury cell model was established to investigate the antioxidant effect of nano-selenium on mouse spermatocyte lines and regulation mechanism expression level activity selenium-containing enzymes induced by stress. safe effective nano-drug system functionalized nanoparticles (SeNPs) developed with lentinan (LNT) (SeNPs@LNT). Mice line GC2-spg cells were treated SeNPs@LNT (1, 2, 4, 8, 16, 32 μM) for 24–72 h evaluate cytotoxicity selenium. randomly divided into following groups: control, hydrogen peroxide (H2O2), SeNPs@LNT, H2O2+SeNPs@LNT groups. group pretreated 4 μM 12 h, followed H2O2 600 8 h. The viability decreased in increased significantly group. Compared group, SeNPs@LNT+H2O2 exhibited obvious red fluorescence, indicating a higher mitochondrial membrane potential. content intracellular reactive oxygen species (ROS) reduced significantly, intensity green fluorescence compared inhibitory generation ROS-induced GPx SOD p53 under intervention nano-selenium, GPx1 increased. In expressions DNA damage-related proteins apoptosis-related than those other Thus, can promote proliferation, improve activities, remove ROS, reduce damage functional abnormalities caused regulating ERK protein levels. has good biological effect, which be used protect male reproductive from